Bacterial Essential Genes Identification

The Geptop 2.0 web server provides an online platform tool to detect essential genes set across bacterial species using http://guolab.whu.edu.cn/geptop/. The nitrogen metabolism gene nucleotides (assimilatory nitrate reductase, respiratory nitrate reductase, nitrite reductase, nitric oxide reductase, hydroxylamine reductase, and glutamine synthetase) of bacteria Lelliottia amnigena were submitted in FASTA format. The web server computed the essentiality score of each gene by comparing Orthology and Phylogeny information (DEG database). The default essentiality cut-off was set at 0.24.

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Thakur P, & Gauba P. (2023). Identification and examination of nitrogen metabolic genes in Lelliottia amnigena PTJIIT1005 for their ability to perform nitrate remediation. BMC Genomics, 24, 104.

Publication 2023

Assimilatory nitrate reductase Bacteria Essential genes Gene Glutamine synthetase Hydroxylamine reductase Lelliottia amnigena Metabolism Nitric oxide reductase Nitrite reductase Nitrogen Nucleotides Respiratory nitrate reductase

Corresponding Organization : Jaypee Institute of Information Technology

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Variable analysis

independent variables

Nitrogen metabolism gene nucleotides (assimilatory nitrate reductase, respiratory nitrate reductase, nitrite reductase, nitric oxide reductase, hydroxylamine reductase, and glutamine synthetase) of bacteria Lelliottia amnigena

dependent variables

Essentiality score of each gene

control variables

Default essentiality cut-off set at 0.24

controls

No positive or negative controls were explicitly mentioned in the information provided.

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