Protocol detail

Plasmid Library for Cell Adhesion Studies

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All plasmids used in this study are available from Addgene (Cambridge, MA). mCherry-Alpha-Actinin2-N-18 (Addgene plasmid #54974), mApple-Lifeact-7 (Addgene plasmid #54747), mEmerald-Lifeact-7 (Addgene plasmid #54148), mCherry-Paxillin-22 (Addgene plasmid #55114) (Paszek et al., 2012 (link)), EGFP-Talin-H-18 (Addgene plasmid #56448), and EGFP-Talin-CW-18 (Addgene plasmid # 56446) were gifts from Michael Davidson.

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Taneja N., Neininger A.C, & Burnette D.T. (2020). Coupling to substrate adhesions drives the maturation of muscle stress fibers into myofibrils within cardiomyocytes. Molecular Biology of the Cell, 31(12), 1273-1288.

Publication 2020

mApple-Lifeact-7 mEmerald-Lifeact-7

Gifts Paxillin Plasmid Talin

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Variable analysis

independent variables

MCherry-Alpha-Actinin2-N-18
MApple-Lifeact-7
MEmerald-Lifeact-7
MCherry-Paxillin-22
EGFP-Talin-H-18
EGFP-Talin-CW-18

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls specified

Annotations

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