Fate-mapping of CCR2+ Monocytes

The wild-type C57BL/6J breeders were purchased from Charles River Laboratories. CCR2^RFP/RFP mice (JAX #017586) and CX3CR1^GFP/GFP (JAX #005582) mice were purchased from the Jackson Laboratory. CCR2^RFP/RFP and CX3CR1^GFP/GFP mice were crossed to obtain CCR2^RFP/+ CX3CR1^GFP/+ pups for most experimental purposes. CCRR2^RFP/+; Actin-GFP mice were derived from CCR2^RFP/RFP and Actin-GFP mice (JAX #003291) for adoptive transfer of monocytes. CCR2-CreER(T2) mice have been characterized 9 (link). The CCR2-CreER mice were crossed with a R26R-GFP Cre-reporter line (Ai6, JAX #007906) or R26R-GFP/Rpl10A mice (JAX #022367). For fate-mapping, the progeny of CCR2-CreER crossed to R26R-GFP or R26R-GFP/Rpl10A mice received 10 mg/kg/day tamoxifen (#T5648, Sigma-Aldrich) at P8 and P9, followed by HI surgery at P10.