SOD (EC 1.15.1.1) and CAT (1.11.1.6) activities were assayed spectrophotometrically following the procedures developed by Beauchamp and Fridovich25 (link) and Zhang et al.26 (link), respectively. The supernatant containing the enzymes was extracted from Allium root tissues by the same procedure proposed by Zou et al.27 . Enzymatic inhibition of photoreduction of nitroblue tetrazolium was followed at a wavelength of 560 nm to determine SOD activity. Enzymatic removal of the hydrogen peroxide (H2O2) substrate was monitored at a wavelength of 240 nm for the estimation of CAT activity. The units of SOD and CAT activities were presented as U/mg FW and OD240nm/min.g FW, respectively.
One of the most distinct bioindicators under stressful conditions inducing oxidative imbalance in the cells is membrane damage, manifested by MDA accumulation. MDA increment resulting from the exposure of the bulbs to test solutions was determined spectrophotometrically following the procedure developed by Heath and Packer28 (link). Derivatized MDA abundances of the samples were monitored at wavelengths of 532 nm and 600 nm. The unit of MDA level was presented as µM/g FW. All procedures for biochemical parameters were repeated three times from beginning to end.
One of the most distinct bioindicators under stressful conditions inducing oxidative imbalance in the cells is membrane damage, manifested by MDA accumulation. MDA increment resulting from the exposure of the bulbs to test solutions was determined spectrophotometrically following the procedure developed by Heath and Packer28 (link). Derivatized MDA abundances of the samples were monitored at wavelengths of 532 nm and 600 nm. The unit of MDA level was presented as µM/g FW. All procedures for biochemical parameters were repeated three times from beginning to end.
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