Identifying and Characterizing LHT Transporters Across Plant Species

In this study, we performed name searches with keywords (e.g., Lysine/Histidine transporter) to obtain annotated candidates of AtLHT and OsLHT family members in the Arabidopsis Information Resource (TAIR, http://www.arabidopsis.org/) (accessed on 25 June 2022) and RAP-DB (http://rapdb.dna.affrc.go.jp/) (accessed on 20 June 2022), respectively. As 10 AtLHT (AtLHT1–10) and 6 OsLHT (OsLHT1-6) transporters have been previously identified, we used the gene nomenclature for Arabidopsis LHTs proposed by Rentsch et al. [4 (link)] and that for rice LHTs proposed by Zhao et al. [5 (link)]. LHT sequences were selected from Physcomitrella patens, Selaginella moellendorffii, Medicago truncatula, Solanum lycopersicum and Sorghum bicolor protein sequences via BLAST searches for Arabidopsis LHTs on the Phytozome v13.1 website (http://www.phytozome.net/) (accessed on 30 June 2022). Information on rice LHT genes, including open reading frame (ORF) lengths, locations and numbers of amino acids were obtained from RAP-DB. The physicochemical parameters of each OsLHT protein were found using ExPASy (http://web.expasy.org/protparam/) (accessed on 20 August 2022). The subcellular localization of the OsLHT proteins was predicted using WoLF PSORT (http://wolfpsort.org) (accessed on 9 September 2022) with the amino acid sequences.