Investigating B. cernua Extract Cytotoxicity on MCF-7 Cells

The MCF-7 human breast cancer cell line was purchased from the ATCC (HTB-22^TM) (transcriptome sequence data were deposited in the ArrayExpress database https://www.ebi.ac.uk/biostudies/arrayexpress/ (accessed on 21 May 2022) by Chiang et al. with accession number E-MTAB-609) [24 (link)]. The cells were grown in Eagle’s minimum essential medium (EMEM) supplemented with 1% penicillin/streptomycin and 10% heat-inactivated fetal bovine serum in a 96-well plate (37 °C, 5% CO₂, 20 h). Then, the cells were treated with various concentrations of B. cernua stem extract (200, 400, 800, and 1600 ppm) for 3, 6, 8, and 24 h. Treatment with solvent (DMSO 0.5% v/v) was also used as a negative control. The cell viability was estimated by measuring absorbance at λ570 nm using a Quant ELISA plate reader (Agilent, CA, USA). Trypan blue staining was used to measure the level of cell death. The morphology of cells was observed using inverted microscopy (Agilent).