Deeply anesthetized rats (see above; n=6 in each group) were placed in a stereotactic apparatus (Stoelting, Kiel, Germany), and the skin overlying the skull was cut open. The lambda and bregma sutures served as landmarks for drilling four holes. FG (Biotium, Hayward, CA) was injected (8 μl of 4% FG in distilled H2O) in the superior colliculus and lateral geniculate bodies [20 (link)-22 (link)]. To allow for the retrograde transport of FG, the animals were kept for 6 days before further experimentation.
Protocol detail
Retrograde Labeling of Visual Pathways
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Corresponding Organization :
Other organizations : Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Dalian Medical University, First Affiliated Hospital of Dalian Medical University
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Variable analysis
independent variables
- Injection of FG (Biotium, Hayward, CA) in the superior colliculus and lateral geniculate bodies
- Retrograde transport of FG
- Deeply anesthetized rats
- Stereotactic apparatus (Stoelting, Kiel, Germany)
- Landmarks: lambda and bregma sutures
- Injection volume: 8 μl of 4% FG in distilled H2O
- Time for retrograde transport: 6 days
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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