Immunofluorescence Staining Protocol

Experiments were performed as described previously (Dong et al., 2013 (link)). Cells were grown on chamber slides, fixed with 4% paraformaldehyde, and incubated with primary antibodies. Secondary antibodies used were Texas red–conjugated goat anti–mouse or FITC-conjugated goat anti–rabbit (Molecular Probe).

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Cao Q., Chen X., Wu X., Liao R., Huang P., Tan Y., Wang L., Ren G., Huang J, & Dong C. (2018). Inhibition of UGT8 suppresses basal-like breast cancer progression by attenuating sulfatide–αVβ5 axis. The Journal of Experimental Medicine, 215(6), 1679-1692.

Publication 2018

Texas red–conjugated goat anti–mouse FITC-conjugated goat anti–rabbit

Antibodies Cells Fitc Goat Molecular probe Mouse Paraformaldehyde Rabbit

Corresponding Organization : Zhejiang University

Other organizations : First Affiliated Hospital Zhejiang University

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Variable analysis

independent variables

Primary antibodies

dependent variables

Cellular localization and expression of target proteins

control variables

Cell culture conditions
Fixation method (4% paraformaldehyde)
Secondary antibodies (Texas red–conjugated goat anti–mouse or FITC-conjugated goat anti–rabbit)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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