Evaluating UCP1 Expression and Hepatic Lipid Content

WAT UCP1 expression was evaluated using anti-UCP1 antibody (1:500; ref. ab10983; Abcam, Cambridge, UK) as previously reported [23 (link),29 (link),30 (link)]. Hepatic lipid content was investigated through oil red O staining, as previously shown [17 (link),24 (link),28 (link),31 (link),32 (link),33 (link)]. The pictures were obtained using a digital camera Olympus XC50 (Olympus Corporation; Tokyo, Japan) at 20×. Digital liver and WAT obtained images were analyzed with ImageJ Software (National Institutes of Health; Bethesda, MD, USA). GFP fluorescence was observed after perfusion of the animals and detected with a fluorescence microscope Olympus IX51 [4 (link),17 (link),23 (link),24 (link),25 (link),26 (link),27 (link),30 (link)].

Free full text: Click here

Liñares-Pose L., Rial-Pensado E., Estévez-Salguero Á., Milbank E., González-García I., Rodríguez C., Seoane-Collazo P., Martinez-Sánchez N., Nogueiras R., Prieto D., Diéguez C., Contreras C, & López M. (2018). Genetic Targeting of GRP78 in the VMH Improves Obesity Independently of Food Intake. Genes, 9(7), 357.

Publication 2018

ab10983

Animals Anti antibody Fluorescence Fluorescence microscope Lipid Liver Perfusion Ucp1

Corresponding Organization : Universidad Complutense de Madrid

Top 5 similar protocols

Variable analysis

independent variables

Anti-UCP1 antibody (1:500; ref. ab10983; Abcam, Cambridge, UK)

dependent variables

WAT UCP1 expression
Hepatic lipid content
GFP fluorescence

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!