Antibodies for Mitochondrial Protein Analysis

The following antibodies were used in this study: rabbit anti-ATOM14 (dilution 1:500), rabbit anti-ATOM69 (dilution 1:50), rabbit anti-ATOM46 (dilution 1:50), rabbit anti-ATOM11 (dilution 1:50)16 (link), rabbit anti-ATOM40 (dilution 1:1000), rabbit anti-VDAC (dilution1:1000), rabbit anti-CoxIV (dilution 1:1000), rabbit anti-cytochrome C (Cyt C) (dilution 1:100), rabbit anti-Tim9 (dilution1:20)34 (link). Mouse anti-c-Myc (Invitrogen, Product No. 132500, dilution 1:2000), mouse anti-HA (Enzo Life Sciences AG, Product No. CO-MMS-R-1000, dilution 1:5000) mouse anti-elongation factor 1a (EF1a) (Merck Millipore, Product No. 05–235, dilution 1:10.000). Rabbit anti-mitochondrial heat shock protein 70 (mtHsp70) (dilution 1:1000) was kindly provided by R. Jensen.

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Mani J., Rout S., Desy S, & Schneider A. (2017). Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei. Scientific Reports, 7, 40738.

Publication 2017

Mouse anti-c-Myc

Antibodies Cytochrome c Dilution Elongation factor Heat shock protein 70 Mitochondrial Mouse Rabbit

Corresponding Organization :

Other organizations : University of Bern

Top 5 similar protocols

Variable analysis

independent variables

Rabbit anti-ATOM14 (dilution 1:500)
Rabbit anti-ATOM69 (dilution 1:50)
Rabbit anti-ATOM46 (dilution 1:50)
Rabbit anti-ATOM11 (dilution 1:50)
Rabbit anti-ATOM40 (dilution 1:1000)
Rabbit anti-VDAC (dilution 1:1000)
Rabbit anti-CoxIV (dilution 1:1000)
Rabbit anti-cytochrome C (Cyt C) (dilution 1:100)
Rabbit anti-Tim9 (dilution 1:20)
Mouse anti-c-Myc (dilution 1:2000)
Mouse anti-HA (dilution 1:5000)
Mouse anti-elongation factor 1a (EF1a) (dilution 1:10.000)
Rabbit anti-mitochondrial heat shock protein 70 (mtHsp70) (dilution 1:1000)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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