Xenograft Studies of Neuroblastoma Cell Lines

The cell lines SH-SY5Y (ATCC CRL-2266) and SK-N-BE(2) (ATCC CRL-2271) were chosen for the xenograft studies. SH-SY5Y is a TP53 wild-type, non-NMYC-amplified cell line that shows no chromosome 1p loss of heterozygosity and is sensitive to chemotherapy. The SK-N-BE(2) cell line shows high resistance to a wide range of chemotherapeutic agents and is characterized by NMYC amplification, TP53 mutation (p.C135F) and chromosome 1p loss of heterozygosity [48 (link)]. Cells were grown in a 1:1 mixture of Eagle's Minimum Essential Medium and Ham’s F12 (Sigma), supplemented with 10% fetal bovine serum (PAA), Glutamax (Gibco), non-essential amino acids (Sigma) and penicillin/streptomycin/amphotericin (Sigma).

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Morscher R.J., Aminzadeh-Gohari S., Feichtinger R.G., Mayr J.A., Lang R., Neureiter D., Sperl W, & Kofler B. (2015). Inhibition of Neuroblastoma Tumor Growth by Ketogenic Diet and/or Calorie Restriction in a CD1-Nu Mouse Model. PLoS ONE, 10(6), e0129802.

Publication 2015

SH-SY5Y SK-N-BE(2 Ham’s F12 Glutamax non-essential amino acids penicillin/streptomycin/amphotericin

Amphotericin Cell line Cells Chemotherapeutic agents resistance Chemotherapy Chromosome Essential amino acids Fetal bovine serum Loss of heterozygosity Mutation Nmyc Penicillin Streptomycin Tp53 Xenograft

Corresponding Organization : Paracelsus Medical University

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Variable analysis

independent variables

Cell lines: SH-SY5Y (ATCC CRL-2266) and SK-N-BE(2) (ATCC CRL-2271)

dependent variables

Xenograft studies

control variables

Cell culture conditions: 1:1 mixture of Eagle's Minimum Essential Medium and Ham's F12 (Sigma), supplemented with 10% fetal bovine serum (PAA), Glutamax (Gibco), non-essential amino acids (Sigma) and penicillin/streptomycin/amphotericin (Sigma)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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