The Staphylococcus aureus WKZ-1 and WKZ-2 strains,10 (link) which are isogenic except for SCCmec type IV, were used in this study. These bacterial strains were obtained through BEI Resources. Both bacterial strains were grown aerobically at 37 °C on 100 mL of tryptic soy broth (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) with shaking at 250 rpm. First, we conducted an minimum inhibitory concentration (MIC) test for oxacillin (Sigma-Aldrich, St. Louis, MO, USA) using the broth microdilution method.11 (link) The MIC values for S. aureus WKZ-1 and WKZ-2 were determined to be 1 mg L−1 and 64 mg L−1, respectively, and we treated with oxacillin equal to 0.5 of the MIC as subinhibitory concentration. oxacillin was treated at the mid-exponential growth phase, and proteome and metabolome sample preparation were performed after 1 h 30 min.
Protocol detail
Comparative Metabolomics of MRSA Strains
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Government of the Republic of Korea
Other organizations : Seoul Institute, Seoul National University, Duksung Women's University, Kumamoto University, Ewha Womans University Medical Center, Ewha Womans University, Konkuk University, Konkuk University Medical Center, Kangdong Sacred Heart Hospital, Hallym University
Top 5 similar protocols
Variable analysis
independent variables
- Oxacillin treatment at 0.5 of the MIC as subinhibitory concentration
- Proteome
- Metabolome
- Bacterial strains: Staphylococcus aureus WKZ-1 and WKZ-2 strains
- Growth conditions: Aerobic growth at 37 °C on 100 mL of tryptic soy broth with shaking at 250 rpm
- Timing of proteome and metabolome sample preparation: After 1 h 30 min of oxacillin treatment
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!