Western blotting was carried out according to the Bio-Rad general protocol (BIO-RAD Bulletin 6376 Rev A). Detailed protocol was previously described 17 (link). Primary antibodies were used as follow: rabbit anti-FOXG1 (Abcam, Cambridge, UK, ab18259, 1:1000); rabbit anti-caspase-9 (#9502, 1:1000), mouse anti-caspase-8 (#9746, 1:1000), rabbit anti-caspase-3 (#9665,1:1000), rabbit anti-cleaved caspase-3 (#9661, 1:1000), rabbit anti-AKT (#9272, 1:1000), mouse anti-ERK1/2 (#4696, 1:1000) and rabbit anti-c-Myc (#5605, 1:1000) were all from cell signaling (Danvers, MA, USA); Mouse anti-GAPDH was purchased from TransGen Biotech and mouse anti-α-tublin was get from Ray antibody Biotech (China).
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Western Blot Analysis of Cell Signaling Pathways
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Corresponding Organization :
Other organizations : Northeast Normal University, First Hospital of Jilin University, Jilin University, Augusta University
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- Primary antibodies used: rabbit anti-FOXG1, rabbit anti-caspase-9, mouse anti-caspase-8, rabbit anti-caspase-3, rabbit anti-cleaved caspase-3, rabbit anti-AKT, mouse anti-ERK1/2, and rabbit anti-c-Myc
- Not explicitly mentioned
- Mouse anti-GAPDH and mouse anti-α-tublin were used as controls
- Positive controls: Mouse anti-GAPDH and mouse anti-α-tublin
- Negative controls: Not explicitly mentioned
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