Automated DNA Extraction and Genotyping

Genomic DNA was extracted from peripheral blood samples using the automated extractor BioRobot EZ1 and the Kit “EZ1 DNA Blood Kit 350μL” (Qiagen Inc., Valencia, CA, USA). Genotyping was performed on an Illumina BeadXpress platform (Illumina, Inc., San Diego, CA, USA) based on Golden Gate chemistry. A 192-plex Illumina VeraCode GoldenGate Genotyping Assay was developed using the “Assay Design Tool” available on the Illumina website [32 ]. A detailed description of the bioinformatic workflow for assay design, analytical procedures, data collection and analysis, and data quality control have been previously published [31 (link)]. Sample replicates were introduced into each analysis. Only the DNA samples and polymorphisms with a call rate > 90% were retained in the final report.