Total protein was extracted from infiltrated systemic leaves as described previously (Li et al., 2014 (link)). Western blotting was performed with primary mouse monoclonal antibody [green fluorescent protein (GFP) or PVX CP protein monoclonal antibody, HuaAn Beijing, China], followed by goat anti-mouse secondary antibody conjugated to alkaline peroxidase (Bio-Rad). Blotted membranes were washed thoroughly and visualized using NBT/BCIP.
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