BMSCs were cultured in 60 mm culture dishes until 60%–70% confluency and further incubated in an adipogenic induction medium (Cyagen Biosciences Inc, China). Cells were maintained in a CO2 incubator (37°C, 5% CO2), and the adipogenic differentiation medium was changed every 3 days. After 28 days of culture, cells were fixed and stained with Oil Red O (Ye et al., 2021 (link)).
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