Microarray Analysis of Total RNA

Total RNA was extracted as described above using Total RNA Purification Kit (Norgen-Biotek Corp.) according to the manufacturer's instructions. One hundred and fifty nanograms of total RNA was labeled and then hybridized to the Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip (Agilent Technologies). All microarray experiments were conducted at the Microarray Core Facility (Stem Cell Unit, King Saud University College of Medicine). Normalization and data analyses were conducted using GeneSpring GX software (Agilent Technologies). Pathway analysis were conducted using the Single Experiment Pathway analysis feature in GeneSpring 12.0 (Agilent Technologies) as described before.^{33 (link), 34 (link)} Twofold cutoff with P<0.02 was used.

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Vishnubalaji R., Hamam R., Abdulla M.H., Mohammed M.A., Kassem M., Al-Obeed O., Aldahmash A, & Alajez N.M. (2015). Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer. Cell Death & Disease, 6(1), e1614-.

Publication 2015

Total RNA Purification Kit Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip GeneSpring GX software GeneSpring 12.0

Chip Human Medicine Microarray Stem cell

Corresponding Organization :

Other organizations : King Saud University, King Khalid University Hospital, University of Southern Denmark

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Variable analysis

independent variables

Total RNA extracted using Total RNA Purification Kit (Norgen-Biotek Corp.) according to the manufacturer's instructions

dependent variables

Gene expression profiles measured using the Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip

control variables

The Microarray Core Facility (Stem Cell Unit, King Saud University College of Medicine) conducted the microarray experiments
Normalization and data analyses were conducted using GeneSpring GX software (Agilent Technologies)
Pathway analysis were conducted using the Single Experiment Pathway analysis feature in GeneSpring 12.0 (Agilent Technologies)

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