Cytokine Quantification Post-SCI

To measure cytokine protein levels by the MSD Mouse Proinflammatory V-Plex Plus Kit (IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, CXCL1, TNF; K15012C, Mesoscale) under naïve conditions and 3 days after SCI, we used a SECTOR Imager 6000 (Mesoscale Discovery, Rockville, USA) Plate Reader according to the manufacturer’s instructions. The same samples as those used for Western blotting were diluted two-fold in Diluent 41 prior to measurement and 50 µl dilution was loaded in each well. Data was analyzed using MSD Discovery Workbench software [50 (link), 51 (link)].

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Ellman D.G., Isaksen T.J., Lund M.C., Dursun S., Wirenfeldt M., Jørgensen L.H., Lykke-Hartmann K, & Lambertsen K.L. (2017). The loss-of-function disease-mutation G301R in the Na+/K+-ATPase α2 isoform decreases lesion volume and improves functional outcome after acute spinal cord injury in mice. BMC Neuroscience, 18, 66.

Publication 2017

MSD Mouse Proinflammatory V-Plex Plus Kit SECTOR Imager 6000

Cxcl1 Cytokine Dilution Ifnγ Il 10 Il 12p70 Il 1β Mouse msd protein

Corresponding Organization : Aarhus University Hospital

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Variable analysis

independent variables

Naïve conditions
3 days after SCI

dependent variables

Cytokine protein levels (IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, CXCL1, TNF)

control variables

Sample dilution (two-fold in Diluent 41)
Sample volume (50 µl dilution loaded in each well)
Measurement technique (SECTOR Imager 6000 Plate Reader)
Analysis software (MSD Discovery Workbench)

controls

Not explicitly mentioned

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