Synthesis of Antimicrobial CHX-HMP Nanoparticles

Aqueous stock solutions of chlorhexidine digluconate and sodium hexametaphosphate (Sigma Aldrich, Gillingham, UK) were mixed in deionized water such that the final concentration was 4 mM CHX and 5 mM HMP. The resulting colloidal suspension of CHX-HMP nanoparticles was mixed thoroughly and then centrifuged at 21000 g for 60 min. The supernatant was removed and discarded and the nanoparticle pellet dried for at least 48 h at 40°C. The pellet was then removed from the centrifuge tubes and ground to a fine white powder composed of nanoparticle aggregates using an agate mortar and pestle. Further details regarding the properties of CHX-HMP nanoparticles and their antimicrobial efficacy can be found elsewhere
[9 (link)].

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Hook E.R., Owen O.J., Bellis C.A., Holder J.A., O’Sullivan D.J, & Barbour M.E. (2014). Development of a novel antimicrobial-releasing glass ionomer cement functionalized with chlorhexidine hexametaphosphate nanoparticles. Journal of Nanobiotechnology, 12, 3.

Publication 2014

chlorhexidine digluconate sodium hexametaphosphate

5 hmp Antimicrobial Chlorhexidine digluconate Powder Sodium hexametaphosphate

Corresponding Organization :

Other organizations : University of Bristol, University Of Bristol Dental Hospital

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Variable analysis

independent variables

Aqueous stock solutions of chlorhexidine digluconate and sodium hexametaphosphate (Sigma Aldrich, Gillingham, UK) were mixed in deionized water

dependent variables

The resulting colloidal suspension of CHX-HMP nanoparticles

control variables

Concentration of CHX (4 mM)
Concentration of HMP (5 mM)

controls

None explicitly mentioned

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