Quantification of Lyso-Gb3 Analogs

Deacylated products (lyso-Gb3 and its eight analogs) were detected using a Xevo TQD MS with an Acquity UPLC system (Waters, Milford, MA), as described previously (27 (link)). All MS parameters were optimized to achieve the highest sensitivity for lyso-Gb3 analogs. Liquid chromatography and MS parameters are summarized in Table S1. The peak areas corresponding to lyso-Gb3 and its analogs in the multiple-reaction-monitoring (MRM) chromatogram were calculated using MassLynx software (Waters).

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Ishii S., Taguchi A., Okino N., Ito M, & Maruyama H. (2020). Determination of globotriaosylceramide analogs in the organs of a mouse model of Fabry disease. The Journal of Biological Chemistry, 295(17), 5577-5587.

Publication 2020

Xevo TQD MS Acquity UPLC system MassLynx software

Liquid chromatography Lyso gb3 Sensitivity

Corresponding Organization : Oita University

Other organizations : Niigata University, Kyushu University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Levels of lyso-Gb3 and its eight analogs

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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