Longitudinal FDG PET/CT Imaging of Tuberculosis

Radiolabeled 2-deoxy-2-(¹⁸F)fluoro-d-glucose (FDG) PET/CT imaging was performed 4, 8 and 12 weeks after Mtb challenge. Imaging was performed using a MultiScan LFER-150 PET/CT scanner (Mediso Medical Imaging Systems) housed within our biosafety level 3 facility as previously described^{66 (link),67 (link)}. Co-registered PET/CT images were analysed using OsiriX MD software (version 12.5.2, Pixmeo) to enumerate granulomas and to calculate the total FDG avidity of the lungs, exclusive of lymph nodes, which are a quantitative measure of total inflammation in the lungs^{66 (link),68}. For historical controls, PET/CT scans were performed using a microPET Focus 220 preclinical PET scanner (Siemens Molecular Solutions) and a clinical eight-slice helical CT scanner (NeuroLogica Corporation)⁶⁸. Thoracic lymphadenopathy and extrapulmonary dissemination of Mtb to the spleen and/or liver were also assessed qualitatively on these scans.

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Larson E.C., Ellis-Connell A.L., Rodgers M.A., Gubernat A.K., Gleim J.L., Moriarty R.V., Balgeman A.J., Ameel C.L., Jauro S., Tomko J.A., Kracinovsky K.B., Maiello P., Borish H.J., White A.G., Klein E., Bucsan A.N., Darrah P.A., Seder R.A., Roederer M., Lin P.L., Flynn J.L., O’Connor S.L, & Scanga C.A. (2023). Intravenous Bacille Calmette–Guérin vaccination protects simian immunodeficiency virus-infected macaques from tuberculosis. Nature Microbiology, 8(11), 2080-2092.

Publication 2023

MultiScan LFER-150 PET/CT scanner OsiriX MD software

Ct scanner Deoxy Glucose Granulomas Helical Inflammation Liver Lungs Lymph nodes Lymphadenopathy Pet ct scans Scans Spleen

Corresponding Organization : University of Pittsburgh

Other organizations : University of Wisconsin–Madison, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Children's Hospital of Pittsburgh

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Variable analysis

independent variables

Time after Mtb challenge (4, 8 and 12 weeks)

dependent variables

Number of granulomas
Total FDG avidity of the lungs (excluding lymph nodes)

control variables

PET/CT imaging equipment used (MultiScan LFER-150 PET/CT scanner and microPET Focus 220 preclinical PET scanner with a clinical eight-slice helical CT scanner)
Biosafety level 3 facility

controls

Historical controls: PET/CT scans performed using a microPET Focus 220 preclinical PET scanner and a clinical eight-slice helical CT scanner

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