Synoviocyte Model for Rheumatoid Arthritis Research

Human synovial sarcoma cell line SW982 is highly recommended as a suitable human synoviocyte model for the study of RA therapy such as fluvastatin-induced apoptosis signaling [17 (link)] and hence was used in this study. The SW982 cell line was cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and incubated at 37°C in humid conditions provided with 5% CO₂. SW982 cells (2 × 10⁵ cells/ml) were plated into 6-well plates overnight before treated with various arthritis-related TLR ligands including 10 ng/ml PGN (TLR2 ligand), 10 μg/ml poly I:C (PIC, TLR3 ligand), 10 ng/ml LPS (TLR4 ligand), and 3 μg/ml imiquimod (TLR7 ligand) and inflammatory cytokines including 100 ng/ml recombinant human IFN-γ (PeproTech, USA), 10 ng/ml recombinant human IL-4 (PeproTech, USA), 10 ng/ml recombinant human TNF-α (PeproTech, USA), and 10 ng/ml recombinant human IL-1β (PeproTech, USA) for various time points. The cells were then harvested for RNA or protein isolation.