Protein Expression Analysis via Western Blot

Western blot analysis was performed as depicted in a previous study (Serna-Salas et al., 2018 (link)). The primary antibodies (GAPDH, Mfn2, and P62 from Abcam; Beclin1, Atg5, and BNIP3 from Cell Signaling Technology; ATP synthase β and LC3 from Sigma) are listed in Supplementary Table S2 online. X-ray film was used to record the protein bands.

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Xue R., Yang J., Jia L., Zhu X., Wu J., Zhu Y, & Meng Q. (2019). Mitofusin2, as a Protective Target in the Liver, Controls the Balance of Apoptosis and Autophagy in Acute-on-Chronic Liver Failure. Frontiers in Pharmacology, 10, 601.

Publication 2019

GAPDH Beclin1 BNIP3

Antibodies Beclin1 Gapdh Mfn2 Protein Synthase Western blot analysis X ray film

Corresponding Organization : Capital Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of GAPDH, Mfn2, P62, Beclin1, Atg5, BNIP3, ATP synthase β, and LC3

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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