Fluorescent Labeling of Forage Grass Genome

Clone 395, derived from the library representing the most frequently present sequences in the F. pratensis genome, was labeled by nick translation with fluorochrome Atto647 (Jena BioScience) (Majka et al. 2017b (link)). The ribosomal sequence 35S rDNA was labeled with digoxigenin-11-dUTP by nick translation. While 5S rDNA and the Arabidopsis thaliana L. telomere repeats (TTTAGGG)_n were labeled by polymerase chain reaction (PCR) with tetramethyl-rhodamine-5-dUTP (Sigma). The total genomic DNA of L. perenne was used as a probe for genomic in situ hybridization and was labeled with digoxigenin-11-dUTP using nick translation kit according to manufacturer instruction (Sigma).

Free full text: Click here

Majka J., Zwierzykowski Z., Majka M, & Kosmala A. (2017). Karyotype reshufflings of Festuca pratensis × Lolium perenne hybrids. Protoplasma, 255(2), 451-458.

Publication 2017

Atto647 tetramethyl-rhodamine-5-dUTP digoxigenin-11-dUTP nick translation kit

Arabidopsis thaliana Clone Digoxigenin 11 dutp Dutp Fluorochrome Genomic In situ hybridization Library Polymerase chain reaction Rdna Ribosomal Telomere Tetramethyl rhodamine

Corresponding Organization : Polish Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Nick translation with fluorochrome Atto647 (Jena BioScience) to label Input protocol: Clone 395, derived from the library representing the most frequently present sequences in the F. pratensis genome
Labeling the ribosomal sequence 35S rDNA with digoxigenin-11-dUTP by nick translation
Labeling 5S rDNA and the Arabidopsis thaliana L. telomere repeats (TTTAGGG)n by polymerase chain reaction (PCR) with tetramethyl-rhodamine-5-dUTP (Sigma)
Labeling the total genomic DNA of L. perenne with digoxigenin-11-dUTP using nick translation kit according to manufacturer instruction (Sigma)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!