The human breast cancer cell lines MDA-MB-231, BT549, Hs578T, HCC1806, and the mouse breast cancer cell line 4T1 were purchased from the American Type Culture Collection (ATCC) and maintained in 10% FBS DMEM media at 37°C and 5% CO2.
The BT549-topotecan resistant line was generated by gradually increasing topotecan concentrations until growing cells became resistant to a topotecan concentration of 1 µM. Resistant cells were then maintained in 10% FBS DMEM media containing 1 µM topotecan at 37°C and 5% CO2.
The Caov3 and Kuramochi ovarian cancer cell lines from the Sandra Orsulic lab at Cedars-Sinai were maintained in 10% FBS RPMI 1640 media at 37°C and 5% CO2.
PDX5 p6, PDX6 p8, and PDX4 p7 56 (link) (from the Julia Unternaehrer lab at Loma Linda University School of Medicine) were maintained in media containing F12 and DMEM with a ratio of 3:1 respectively, 5% FBS, 0.4 µg/mL hydrocortisone, 5 µg/mL insulin, 8.4 ng/mL cholera toxin, 24 µg/mL adenine at 37°C and 5% CO2.
The BT549-topotecan resistant line was generated by gradually increasing topotecan concentrations until growing cells became resistant to a topotecan concentration of 1 µM. Resistant cells were then maintained in 10% FBS DMEM media containing 1 µM topotecan at 37°C and 5% CO2.
The Caov3 and Kuramochi ovarian cancer cell lines from the Sandra Orsulic lab at Cedars-Sinai were maintained in 10% FBS RPMI 1640 media at 37°C and 5% CO2.
PDX5 p6, PDX6 p8, and PDX4 p7 56 (link) (from the Julia Unternaehrer lab at Loma Linda University School of Medicine) were maintained in media containing F12 and DMEM with a ratio of 3:1 respectively, 5% FBS, 0.4 µg/mL hydrocortisone, 5 µg/mL insulin, 8.4 ng/mL cholera toxin, 24 µg/mL adenine at 37°C and 5% CO2.
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