Wheat Genome Structural Rearrangements

Example 2

A 30× genome coverage of both Xi19 and Robigus was generated. Bio-informatic mapping of the gDNA reads to the IWGSCWGAV02_2BS_scaffold14096, IWGSCWGAV02_2BS_scaffold11627 and the BAC sequence confirmed large structural differences in the region, which explain the observed lack of recombination. Essentially, there were no gDNA-seq reads from Xi19 that mapped onto the BAC sequence of Renan (FIG. 3). Similarly, none of the gDNA-seq reads from Robigus mapped onto scaffold11627 portion located in the interval. This indicates that the gene content of the corresponding genomic regions in susceptible and resistant plants is very divergent and this was probably caused by a local chromosomal rearrangement. Similar structural rearrangements between the Chinese Spring reference genome sequence and the region containing the Fusarium head blight resistance gene (Fhb1) have recently been published (Schweiger et al, 2016).