T Cell Phenotype Analysis After Infection

Four days after infection, T cells were harvested and washed once with PBS, stained with rabbit anti-FLT3L antibody (Abcam, USA) for 1 h at 4 °C, and washed twice. Then PE donkey anti-rabbit IgG antibody (Biolegend, USA) was added, incubated at 4 °C for 30 min, and analyzed by flow cytometry using CantoII flow cytometer (BD Biosciences, San Jose, CA, USA) [14 (link)]. For T cell phenotype analysis, T cells were harvested 7 days after infection and washed once with PBS, stained with anti-CD4-PE/Cy7 (Biolegend, USA), anti-CD8-PerCP-Cy5.5 (Biolegend, USA), anti-CCR7-PE (Biolegend, USA), and anti-CD45RA-Pacific Blue (Biolegend USA) 30 min at 4 °C, then washed and resuspended in PBS for flow cytometry analysis [15 (link)].