Nanoparticle production by pulsed picosecond laser ablation in deionised water (dH2O) was described in our previous publication [21 (link)]. Briefly, Ag plates (dimensions of 25 mm × 25 mm × 2 mm, purity 99.99%) were sterilised by immersion into ethanol and then autoclaved with dH2O. The Ag plates were then placed into a glass vessel containing 20 mL of dH2O at a level of 2 mm above them. A picosecond pulsed Nd: YVO4 laser with a wavelength of 1064 nm was used to ablate the plate at a pulse repetition rate of 200 kHz and an average power of 9.12 W. The average size for AgNPs produced was 27.2 nm, ranging from 10–70 nm [21 (link)].
The chemically produced AgNPs (concentration 20 μg/mL and average size about 35 nm) were purchased from Sigma-Aldrich (Dorset, UK).
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