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Chromatin Immunoprecipitation Sequencing Protocol

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Fontanals-Cirera B., Hasson D., Vardabasso C., Di Micco R., Agrawal P., Chowdhury A., Gantz M., de Pablos-Aragoneses A., Morgenstern A., Wu P., Filipescu D., Valle-Garcia D., Darvishian F., Roe J.S., Davies M.A., Vakoc C.R., Hernando E, & Bernstein E. (2017). Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Molecular cell, 68(4), 731-744.e9.
Publication 2017
Antibodies Antibody Brd4 Buffer Bulk Cells Chip Chip seq Chloroform Chromatin Cold Ctcf Deoxycholic acid Digestion Dilution Dna polymerase Edta Egta Enzymes Glycine H3k4me3 Hepes Ip 20 Library Med1 Mgcl2 Ml iii Mouse Nacl Nahco3 Np 40 Nuclei Nucleosomes Pellets Phenol Polynucleosomes Protease inhibitor Protein a Proteinase k Rabbit Rnase Sensitivity Sucrose Tris Tween Tween 20

Corresponding Organization : Icahn School of Medicine at Mount Sinai

Other organizations : Cold Spring Harbor Laboratory, The University of Texas MD Anderson Cancer Center

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