An Nkx2.5Cre/+ knock-in mouse line was kindly provided by Dr. Robert Schwartz14 (link). An αMHCCre/+ transgenic mouse line was kindly provided by Dr. E. Dale Abel15 (link). ROSA26eGFP-DTA and ROSA26LacZ mouse lines were purchased from the Jackson Laboratory16 (link), 17 (link). Experimental animal protocols were approved by the Institutional Animal Care and Use Committees of Massachusetts General Hospital and Stanford University. All experiments were performed on somite-matched embryos or sex-matched adult mice.
Protocol detail
Genetic Mouse Lines for Cardiac Research
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Adult
Embryos
Experimental animal
Institutional animal care and use committees
Mice
Somite
Transgenic mouse
Corresponding Organization :
Other organizations : Massachusetts General Hospital, Cardiovascular Institute of the South, Stanford University
Top 5 similar protocols
Variable analysis
independent variables
- Nkx2.5Cre/+ knock-in mouse line
- αMHCCre/+ transgenic mouse line
- Not explicitly mentioned
- ROSA26eGFP-DTA mouse line
- ROSA26LacZ mouse line
- Sex-matched adult mice
- Somite-matched embryos
- Positive control: ROSA26eGFP-DTA and ROSA26LacZ mouse lines
- Negative control: Sex-matched adult mice and somite-matched embryos
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