Intracerebral Hemorrhage Mouse Model

The ICH model was conducted according to our previously developed method (Yuan et al., 2019 (link)). Briefly, mice were immobilized on the stereotaxic apparatus (RWD Life Science Co, Shenzhen, China) after being anesthetized with 3% isoflurane for induction and 1.5% for maintenance. Whole blood (20 μl) without anticoagulant was obtained from its tail and then injected into the left striatum (0.8 mm anterior and 2 mm lateral of bregma, at a depth of 3.5 mm) using a syringe pump (Hamilton, Bonaduz, AG) at 2.0 μl/min. The microinjector was detained for 10 min. The sham group was injected with 20 μl saline using the same procedures. Unsuccessful ICH models that were asymptomatic or dead were excluded from this study. Perihematomal cerebral tissues were collected for oxylipins quantification at 0.5, 1, and 3 days after ICH or 1 day after saline injection.