Midbrain dopamine differentiation from hESCs
Corresponding Organization : Stony Brook University
Other organizations : Rockefeller University, Memorial Sloan Kettering Cancer Center, Stony Brook School, Baylor College of Medicine
Variable analysis
- Cell line (WT H9 (WA-09), SATB1^KO, NURR1-GFP reporter line)
- Midbrain dopamine (DA) differentiation
- Maintenance of hESCs using E8-essential medium without feeder on VTA-N
- Passaging of hESCs every 4-5 days by EDTA
- Positive control: Previously published protocol for midbrain dopamine differentiation from hESCs (Kim et al., 2021; Riessland et al., 2019)
- Negative control: Not explicitly mentioned
Annotations
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