The hESCs WT H9 (WA‐09), SATB1KO, NURR1‐GFP reporter line were maintained using E8‐essential medium (Fisher Scientific) without feeder on VTA‐N (Fisher Scientific) and passaged every 4–5 days by EDTA. Midbrain dopamine (DA) differentiation from hESC was done with a protocol previously published by our group (Kim et al., 2021 (link); Riessland et al., 2019 (link)).
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