Plasma Sex Steroid Quantification

Concentrations of sex steroids on plasma samples were measured using commercial testosterone (IBL America, Minneapolis, MN) and estradiol (Calbiotech Inc., El Cajon, CA) ELISA kits, as per the manufacturer’s instructions (3 (link), 10 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Dhakal S., Park H.S., Seddu K., Lee J., Creisher P.S., Davis K.M., Hernandez I.R., Maul R.W, & Klein S.L. (2023). Estradiol Mediates Greater Germinal Center Responses to Influenza Vaccination in Female than Male Mice. bioRxiv.

Publication Preprint 2023

estradiol

Corresponding Organization : Johns Hopkins University

Other organizations : National Institute on Aging, National Institutes of Health

Top 5 similar protocols

Variable analysis

independent variables

Concentrations of sex steroids

dependent variables

Plasma samples

control variables

Commercial testosterone (IBL America, Minneapolis, MN) and estradiol (Calbiotech Inc., El Cajon, CA) ELISA kits, as per the manufacturer's instructions

controls

Positive controls and negative controls not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!