Colon Tissue Analysis: Molecular Markers

Four-micron-thick sections were prepared from formalin-fixed, paraffin-embedded colon tissue from AOM/DSS-treated mice and stained with H&E. Immunohistochemical staining against BrdU, PCNA, IL-1β, IL-6, NF-κB and Nrf2 was performed with standard techniques. Image pro plus software was used to analyze the number of positive cells by detecting IOD.

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Yao J., Zhao L., Zhao Q., Zhao Y., Sun Y., Zhang Y., Miao H., You Q.D., Hu R, & Guo Q.L. (2014). NF-κB and Nrf2 signaling pathways contribute to wogonin-mediated inhibition of inflammation-associated colorectal carcinogenesis. Cell Death & Disease, 5(6), e1283-.

Publication 2014

Brdu Colon Embedded paraffin Formalin Il 1β Mice Nf κb Nrf2 Pcna

Corresponding Organization :

Other organizations : China Pharmaceutical University

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