Female Balb/c mice at 6–8 weeks of age were used for LPS experiments (Charles River Labs). Ripk3−/− (on C57BL/6 background) and matched controls were previously described (Newton et al., 2004) and provided to us by Dr. Vishva Dixit (Genentech). Sting−/− mice were a gift from Dr. Alexander Poltorak. TRIF−/−(Ticam1−/−) (C57BL/6J-Ticam1Lps2/J) mice and corresponding control mice (C57BL/6J, B6.129P2) were purchased from Jackson labs. D138N RIPK1, K45A RIPK1, K51A RIPK3, and Mlkl−/− mice were previously described (30 (link), 32 –34 ).
All use of animals was approved by the Tufts University, UMASS, and Fox Chase Cancer Center Institutional Animal Care and Use Committees. Mice were maintained in Tufts animal facility in cages with light/dark cycle and experiments performed according to the protocol with all efforts to minimize the number and suffering of the animals.
All use of animals was approved by the Tufts University, UMASS, and Fox Chase Cancer Center Institutional Animal Care and Use Committees. Mice were maintained in Tufts animal facility in cages with light/dark cycle and experiments performed according to the protocol with all efforts to minimize the number and suffering of the animals.
