Orthotopic Patient-Derived Cervical Cancer Model

The 57-year-old patient with primary cervical cancer previously received a radical hysterectomy with bilateral salpingo-oophorectomy and pelvic lymphadenectomy at Kawasaki Medical School Hospital, Japan [9 (link)]. The tumor was diagnosed as squamous cell carcinoma (grade 2). The patient did not receive any neoadjuvant therapy. Informed consent was obtained, and PDOX studies were approved by the Institutional Ethics Committee of Kawasaki Medical School. A fresh resected tumor specimen was initially implanted sub-cutaneously in nude mice. The established tumors were cut into 5 mm³ fragments for surgical orthotopic implantation (SOI). A 6 mm lower abdominal midline incision was made under anesthesia. The neck of the uterus was exposed, and a single fragment was implanted by SOI using 8–0 nylon sutures (Ethicon, Inc., NJ, USA). The wound was closed with 6–0 PDS II (polydioxanone) sutures (Ethicon, Inc., NJ, USA) [23 (link)–25 (link)].