ChIP on EP300 was performed as described previously (Jacobs et al. 2014 (link)), with an excess of EP300 antibody (C-20; sc-585 X; Lot B0211 and Lot E2610). For H3K27ac and H3K4me3 data, ChIP was based on Vermunt et al. (2014) (link), with 5 µg H3K27ac (Abcam ab4729, Lot GR3303561-2) and 6 µg H3K4me3 (Millipore 07-473, Lot 3394198) per sample. ChIP-seq data analysis on EP300 was performed as described previously (Jacobs et al. 2014 (link)). For the full procedure, see Supplemental Material .
Protocol detail
ChIP-seq for EP300, H3K27ac, and H3K4me3
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Corresponding Organization :
Other organizations : Netherlands Institute for Neuroscience, University of Amsterdam, Amsterdam University Medical Centers, Vrije Universiteit Amsterdam, Amsterdam Neuroscience, University of Queensland, Mater Research, University of California, Santa Cruz, Howard Hughes Medical Institute, Delft University of Technology
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Variable analysis
independent variables
- EP300 antibody (C-20; sc-585 X; Lot B0211 and Lot E2610)
- H3K27ac antibody (Abcam ab4729, Lot GR3303561-2)
- H3K4me3 antibody (Millipore 07-473, Lot 3394198)
- ChIP-seq data on EP300
- ChIP-seq data on H3K27ac
- ChIP-seq data on H3K4me3
- Experimental procedures for ChIP on EP300 as described in Jacobs et al. 2014
- Experimental procedures for ChIP on H3K27ac and H3K4me3 as described in Vermunt et al. 2014
- Positive control: None specified
- Negative control: None specified
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