IHC and IF were performed as previously described (31 (link)). In brief, mice were deeply anesthetized and transcardially perfused with PBS and 4% paraformaldehyde (PFA). Brains were excised, post fixed in 4% PFA over night, saturated in 30% sucrose solution, and sectioned with a cryotome at 30 μm. IHC staining of microglia was performed using anti-IBA1 antibody (Wako Chemicals, Neuss, Germany) and imaged using a Zeiss AXIO Scope A1 equipped with a Plan-APOCHROMAT 20x/0.8 objective. Images were acquired at room temperature with the AxioRel Vision 4.8 software and quantified in ImageJ. For double IF goat anti-GFAP antibody (Sigma Aldrich, St. Louis, USA) and rabbit anti-MGL antibody (kind gift from Ken Mackie, Indiana, USA) and anti-goat (Alexa 488 conjugated) and anti-rabbit (Alexa 594 conjugated) secondary antibodies were used. Sections were imaged by confocal laser scanning microscopy using a Leica TCS SP5 II equipped with a HCX PL APO 63 × 1.3 NA objective. Images were obtained at room temperature with Leica LAS AF software.