An expired unit of donor packed red blood cells was acquired from the UC
Davis Medical Center hospital transfusion services, and the provision approved
by the Department of Pathology Clinical Research Oversight Committee Internal
Review. RBC membrane was prepared according to previous studies with
modification16 , 34 (link). Briefly, the RBCs were lysed
in the hypotonic medium (0.25×PBS) for 90 mins on ice. Samples were
centrifuged at 80000 × g for 90 min with a Beckman L7-65 Ultracentrifuge.
The supernatant was removed and the pink pellet was re-suspended in water. The
membrane protein concentrations were quantified using BCA protein assay kit
(Pierce, Rock-ford, IL).
Davis Medical Center hospital transfusion services, and the provision approved
by the Department of Pathology Clinical Research Oversight Committee Internal
Review. RBC membrane was prepared according to previous studies with
modification16 , 34 (link). Briefly, the RBCs were lysed
in the hypotonic medium (0.25×PBS) for 90 mins on ice. Samples were
centrifuged at 80000 × g for 90 min with a Beckman L7-65 Ultracentrifuge.
The supernatant was removed and the pink pellet was re-suspended in water. The
membrane protein concentrations were quantified using BCA protein assay kit
(Pierce, Rock-ford, IL).
