LOX enzyme
was extracted from pig skin by the method of Shackleton and Hulmes.24 (link) LOX catalytic activity was determined using
a horseradish peroxidase (HRP)-coupled fluorescent assay previously
described,19 (link) with cadaverine hydrochloride
as a substrate, BAPN as positive control, and a preincubation time
of 20 min with nine dilutions from a top concentration of 100 μM.
LOXL2 was purchased from R&D System. LOXL2 catalytic activity
was determined using the Promega ROS-Glo assay kit with cadaverine
hydrochloride as a substrate, BAPN as positive control, and a preincubation
time of 20 min at the same concentrations as above.
was extracted from pig skin by the method of Shackleton and Hulmes.24 (link) LOX catalytic activity was determined using
a horseradish peroxidase (HRP)-coupled fluorescent assay previously
described,19 (link) with cadaverine hydrochloride
as a substrate, BAPN as positive control, and a preincubation time
of 20 min with nine dilutions from a top concentration of 100 μM.
LOXL2 was purchased from R&D System. LOXL2 catalytic activity
was determined using the Promega ROS-Glo assay kit with cadaverine
hydrochloride as a substrate, BAPN as positive control, and a preincubation
time of 20 min at the same concentrations as above.
