Protocol detail

Metabolic Biomarker Analysis in Mice

Find Similar Protocols

Hitachi 7180 auto-analyzers were used to analyze serum total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterols, triglycerides, non-esterified fatty acids (NEFA), and ketone body concentrations at Oriental Yeast Co., Ltd. (Shiga, Japan). Enzyme-linked immunosorbent assay (ELISA) was used to measure serum insulin (mouse insulin ELISA kit, Shibayagi Co., Ltd., Gunma, Japan) and sIgA concentrations (ELISA Kit for Secretory Immunoglobulin A, Cloud-Clone Corp., Katy, TX, USA). Hepatic cholesterol and triglyceride levels were analyzed according to a previous report [20 (link),25 (link)].

Free full text: Click here

Aoe S., Yamanaka C, & Mio K. (2021). Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice. Nutrients, 13(10), 3406.

Publication 2021

7180 auto-analyzers ELISA Kit for Secretory Immunoglobulin A

Cholesterol Clone Enzyme linked immunosorbent assay Esterified fatty acids High density lipoprotein cholesterols Insulin Ketone body Low density lipoprotein Mouse Oriental Secretory immunoglobulin a Serum Triglyceride Yeast

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum total cholesterol
Serum low-density lipoprotein (LDL) cholesterol
Serum high-density lipoprotein (HDL) cholesterol
Serum triglycerides
Serum non-esterified fatty acids (NEFA)
Serum ketone body concentrations
Serum insulin
Serum secretory immunoglobulin A (sIgA)
Hepatic cholesterol
Hepatic triglyceride levels

control variables

Hitachi 7180 auto-analyzers used to analyze serum parameters
Enzyme-linked immunosorbent assay (ELISA) used to measure serum insulin and sIgA
Hepatic cholesterol and triglyceride levels analyzed according to previous reports

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!