Antiviral agents included two active site protease inhibitors telaprevir (Selleckchem, Houston, TX, USA) and boceprevir (ChemScene, Monmouth Junction, NJ, USA), NS5A inhibitor daclatasvir (Selleckchem), two nucleotide NS5B polymerase inhibitors, sofosbuvir (Advanced Chemblocks, Burlingame, CA, USA) and 2′-C-methylcytidine (US Biological, Salem, MA, USA), host-targeting cyclophillin inhibitor cyclosporin A (Sigma-Aldrich, St Louis, MO, USA) and entry-inhibitor (S)-chlorcyclizine (NCATS, Bethesda, MD, USA).
The HCVcc-Luc infectious virus consisted of a full-length J6/JFH-1 HCV with insertion of a luciferase reporter gene at the 3′ end of the p7 gene [16 (link)].
Huh7.5.1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Serum Source International, Charlotte, NC, USA), 100 IU/ml penicillin, and 100 μg/ml streptomycin in 5% CO2, at 37°C. A stably expressing Con1b replicon cell line with luciferase reporter under the direction of 5′-NTR for quantification described previously [21 (link),22 (link)] was grown in the same condition as Huh7.5.1 cells with the addition of 500 μg/ml G-418.
The HCVcc-Luc infectious virus consisted of a full-length J6/JFH-1 HCV with insertion of a luciferase reporter gene at the 3′ end of the p7 gene [16 (link)].
Huh7.5.1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Serum Source International, Charlotte, NC, USA), 100 IU/ml penicillin, and 100 μg/ml streptomycin in 5% CO2, at 37°C. A stably expressing Con1b replicon cell line with luciferase reporter under the direction of 5′-NTR for quantification described previously [21 (link),22 (link)] was grown in the same condition as Huh7.5.1 cells with the addition of 500 μg/ml G-418.
