Purification of His-tagged IL-15/IL-15Rα

We generated a pDisplay Vector (Life Technologies) encoding His-tagged IL-15/IL-15Rα (IL-15 linked to IL-15P sushi)^{28 (link)}. Following transfection of 293-F cells, we purified protein from culture supernatant using standard Cobalt agarose resin (Thermo Scientific) according to protocol.

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Stephan S.B., Taber A.M., Jileaeva I., Pegues E.P., Sentman C.L, & Stephan M.T. (2014). Biopolymer implants enhance the efficacy of adoptive T cell therapy. Nature biotechnology, 33(1), 97-101.

Publication 2014

pDisplay Vector Cobalt agarose resin

Agarose Cells Cobalt Il 15 Protein Resin Transfection Vector

Corresponding Organization :

Other organizations : Fred Hutch Cancer Center, Technology Access Foundation, Dartmouth College

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Variable analysis

independent variables

Transfection of 293-F cells with pDisplay Vector (Life Technologies) encoding His-tagged IL-15/IL-15Rα (IL-15 linked to IL-15P sushi)

dependent variables

Purification of protein from culture supernatant using standard Cobalt agarose resin (Thermo Scientific) according to protocol

control variables

Protein purification protocol

controls

No positive or negative controls were explicitly mentioned.

Annotations

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