Within subjects, we performed a before and after study instead of a cross over study given that the necessary washout period of ivermectin in chinchillas is unknown. In this experiment, the 10 chinchillas were injected subcutaneously with ivermectin injection (Ivomec®, Merial, Ingelheim am Rhein, Germany, 1.0% w/v sterile solution, dilute 10 times by saline) 0.4 mg/kg on day 0.
Fresh fecal samples were collected on day 0 and day 14, divided into day 0 group and day 14 group, respectively. Chinchillas were placed individually into clean cages, and fecal pellets were collected upon evacuation. Following collection, these samples were transferred immediately into sterile cryogenic vials by sterile forceps and stored at −80 °C until microbiota analysis [14 (link)].
Fresh fecal samples were collected on day 0 and day 14, divided into day 0 group and day 14 group, respectively. Chinchillas were placed individually into clean cages, and fecal pellets were collected upon evacuation. Following collection, these samples were transferred immediately into sterile cryogenic vials by sterile forceps and stored at −80 °C until microbiota analysis [14 (link)].
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