Comet Assay for DNA Damage Detection

A DNA damage assay was carried out using an OxiSelect Comet Assay kit (STA-351, Cell Biolabs, San Diego, CA, USA). Briefly, cells were seeded in a 6-well plate, treated with or without BP (MDA-MB-231: 50 μg/mL, MCF-7: 75 μg/mL) for 24 h, and then exposed to 6 Gy IR. After 48 h, the cells were harvested and the comet assay performed according to the manufacturer’s instructions. The comet tails were imaged using an Olympus CKX41 fluorescence microscope at ×400 magnification. The images were analyzed using OpenComet software (v1.3.1, OpenComet is an open-source software tool providing automated analysis of comet assay images. The OpenComet plug-in and source code is available at www.opencomet.org.) [26 (link)].