Intracerebroventricular drug administration in SAH

Intracerebroventricular drug administration was performed as reported previously^{20 (link)–21 (link)}. A burr hole was drilled into the skull according to the following coordinates relative to bregma: 1.5 mm posterior and 1.0 mm lateral. The needle of a 10µL Hamilton syringe (Microliter 701; Hamilton Company, Reno, NV) was inserted through the burr hole into the left lateral ventricle through the burr hole 4.0 mm below the horizontal plane of bregma. Sterile PBS vehicle or 5ul rOPN (0.1ng in 5ul; EMD Chemicals, La Jolla, CA) were administered 3 hours after SAH induction by a pump at a rate of 0.5 ul /min., respectively. GRGDSP (Sigma-Aldrich, St. Louis, MO; 100pmol in 1uL), Fib-14 (Tocris Bioscience, Ellisville, MI; 0.2 mg in 5 µL PBS) or NSC23766 (Santa Cruz Biotechnology; 30ug/5ul) was administered 1 hour before SAH induction. 500 pmol/5ul ILK siRNA or scrambled siRNA (Life Technologies) was infused at the same rate 48 hours before SAH modeling.

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Wu J., Zhang Y., Yang P., Enkhjargal B., Manaenko A., Tang J., Pearce W.J., Hartman R., Obenaus A., Chen G, & Zhang J.H. (2016). rOsteopontin Stabilizes Smooth Muscle Cell Phenotype via Integrin Receptor/ILK/Rac-1 Pathway after SAH in Rats. Stroke; a journal of cerebral circulation, 47(5), 1319-1327.

Publication 2016

Microliter 701 GRGDSP Fib-14 NSC23766 scrambled siRNA

Burr hole Grgdsp Lateral ventricle Needle Nsc23766 Sirna Skull Sterile Syringe

Corresponding Organization :

Other organizations : First Affiliated Hospital of Soochow University, Soochow University, Loma Linda University

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Variable analysis

independent variables

Intracerebroventricular administration of sterile PBS vehicle or 5ul rOPN (0.1ng in 5ul)
Intracerebroventricular administration of GRGDSP (100pmol in 1uL), Fib-14 (0.2 mg in 5 µL PBS), or NSC23766 (30ug/5ul) 1 hour before SAH induction
Intracerebroventricular infusion of 500 pmol/5ul ILK siRNA or scrambled siRNA 48 hours before SAH modeling

dependent variables

Not explicitly mentioned

control variables

Burr hole coordinates relative to bregma: 1.5 mm posterior and 1.0 mm lateral, needle inserted 4.0 mm below the horizontal plane of bregma
Pump rate of 0.5 ul /min for intracerebroventricular administration

controls

Positive control: Sterile PBS vehicle
Negative control: Scrambled siRNA

Annotations

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