Inflammatory Marker Measurements in Fasting Blood

Fasting blood samples were collected at the study centre and were stored at −80 °C until full blood count measurements. These measurements included absolute counts of granulocytes, lymphocytes and platelets and were performed using the COULTER® Ac·T diff2™ Hematology Analyzer (Beckman Coulter, San Diego, California, USA). In an additional analysis, the normal distribution of hemoglobin and CRP levels were assessed as well. CRP levels were measured using a particle enhanced immunoturbidimetric assay (Roche Diagnostics, Mannheim, Germany).
The neutrophil-to-lymphocyte ratio was calculated on the basis of absolute peripheral granulocyte (as a proxy for the neutrophil count) (N; ×10⁹/Liter) and lymphocyte (L; ×10⁹/Liter) blood counts, using the formula: NLR = N/L^{9 (link)}.
The platelet-to-lymphocyte ratio was calculated on the basis of peripheral platelet(P; ×10⁹/Liter) and lymphocyte (L; ×10⁹/Liter) blood counts, using the formula: PLR = P/L^{12 (link)}.
The systemic immune-inflammation index (SII) was calculated on the basis of peripheral platelet (P; ×10⁹/Liter), granulocyte (N; ×10⁹/Liter) and lymphocyte (L; ×10⁹/Liter) blood counts, using the following formula: SII = P * N/L^{10 (link)}. All the inflammatory markers are either ratios or indices and as such do not have a unit.

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Fest J., Ruiter R., Ikram M.A., Voortman T., van Eijck C.H, & Stricker B.H. (2018). Reference values for white blood-cell-based inflammatory markers in the Rotterdam Study: a population-based prospective cohort study. Scientific Reports, 8, 10566.

Publication 2018

COULTER® Ac·T diff2™ Hematology Analyzer particle enhanced immunoturbidimetric assay

Blood Diagnostics Full blood count Granulocyte Hemoglobin Immunoturbidimetric assay Inflammatory Lymphocyte Neutrophil Peripheral platelet

Corresponding Organization :

Other organizations : Erasmus MC

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Absolute counts of granulocytes, lymphocytes and platelets
Hemoglobin levels
CRP levels
Neutrophil-to-lymphocyte ratio (NLR)
Platelet-to-lymphocyte ratio (PLR)
Systemic immune-inflammation index (SII)

control variables

Fasting blood samples were collected at the study centre
Blood samples were stored at −80 °C until full blood count measurements

positive controls

None mentioned

negative controls

None mentioned

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