Modeling Preterm Birth in Mice

Male and female C57BL/6 mice were bred overnight, and copulation plug was identified in the morning and defined as 0.5 dpc. On embryonic day (E) 15.5, dams were given ketamine and xylazine anesthesia and a mini- laparotomy was performed. Ultrapure LPS (E. coli O111:B4, Invivogen), 250 ug (as originally described by Lyttle et al, (29 ), or 10 ug (as described by Edey et al (30 (link)), in a 100 μL volume or sterile saline solution was infused between 2 gestational sacs in the lower right uterine horn. Uterus was then returned to abdomen; the fascia and the skin was sutured. The entire procedure took about 5 minutes per mouse. The mice were observed under infrared-camera for preterm birth. IRAK1 inhibitor (407601, Sigma-Aldrich) was given 4 hours before the surgery. Mice genetically deficient for IRAK-1 were created on a C57BL/6 background as previously described (26 (link)). For mechanistic studies, mice were sacrificed 12 hours after LPS injection.

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Jain V.G., Kong F., Kallapur S.G., Presicce P., Senthamaraikannnan P., Cappelletti M., Chougnet C.A., Bhattacharyya S., Pasare C, & Muglia L.J. (2020). IRAK1 is a critical mediator of inflammation induced preterm birth. Journal of immunology (Baltimore, Md. : 1950), 204(10), 2651-2660.

Publication 2020

E. coli O111:B4

Abdomen Anesthesia C57bl mice E coli Embryonic Fascia Female Gestational sacs Irak 1 Ketamine Laparotomy Male Mice Preterm birth Saline solution Skin Sterile Surgery Uterine horn Uterus Xylazine

Corresponding Organization : Cincinnati Children's Hospital Medical Center

Other organizations : University of California, Los Angeles, University of Cincinnati Medical Center

Top 5 similar protocols

Variable analysis

independent variables

Dose of LPS (250 ug or 10 ug)
Administration of IRAK1 inhibitor (4 hours before surgery)

dependent variables

Preterm birth

control variables

C57BL/6 mouse strain
Embryonic day (E) 15.5
Laparotomy procedure
Volume of LPS or saline solution (100 μL)
Surgical duration (5 minutes per mouse)
Observation under infrared-camera for preterm birth

positive controls

Mice genetically deficient for IRAK-1

negative controls

Sterile saline solution

Annotations

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