Comprehensive Antibody Panel for Muscle Analysis

The following primary antibodies were used: rabbit polyclonal anti-MURC/cavin-4 for immunohistochemical analysis (Code: HPA020973, Sigma-Aldrich, Milan, Italy); goat polyclonal anti-MURC/cavin-4 for immunoblotting analysis (Code: SC-163021, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit polyclonal anti-MURC/cavin-4 for immunofluorescence analysis (as described in [52 (link)]); mouse monoclonal anti-Cav-3 (Code: 610420, BD Transduction Laboratories, Buccinasco, Milan, Italy); rabbit anti-myogenin (Santa Cruz Biotechnology, Dallas, TX, USA, 1:500 dilution); mouse monoclonal anti-MHC (Code: SC-32732, Santa Cruz Biotechnology, Dallas, TX, USA); mouse monoclonal anti-total and—phosphorylated ERK1/2 (extracellular regulated kinase 1/2) (Tyr204) (Code: SC-135900 and SC-7383, Santa Cruz Biotechnology, Dallas, TX, USA); mouse monoclonal anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (Code: MAB374, Millipore, Darmstadt, Germany) and mouse monoclonal anti-alpha-tubulin (Code: T5168, Sigma-Aldrich, Milan, Italy).

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Faggi F., Codenotti S., Poliani P.L., Cominelli M., Chiarelli N., Colombi M., Vezzoli M., Monti E., Bono F., Tulipano G., Fiorentini C., Zanola A., Lo H.P., Parton R.G., Keller C, & Fanzani A. (2015). MURC/cavin-4 Is Co-Expressed with Caveolin-3 in Rhabdomyosarcoma Tumors and Its Silencing Prevents Myogenic Differentiation in the Human Embryonal RD Cell Line. PLoS ONE, 10(6), e0130287.

Publication 2015

rabbit anti-myogenin mouse monoclonal anti-MHC mouse monoclonal anti-GAPDH mouse monoclonal anti-alpha-tubulin

Alpha tubulin Antibodies Dilution Erk1 Gapdh Glyceraldehyde 3 phosphate dehydrogenase Goat Immunoblotting analysis Immunofluorescence Kinase Mouse Myogenin Rabbit

Corresponding Organization : Oregon Health & Science University

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal anti-MURC/cavin-4
Goat polyclonal anti-MURC/cavin-4
Rabbit polyclonal anti-MURC/cavin-4
Mouse monoclonal anti-Cav-3
Rabbit anti-myogenin
Mouse monoclonal anti-MHC
Mouse monyclonal anti-total and—phosphorylated ERK1/2 (extracellular regulated kinase 1/2) (Tyr204)
Mouse monoclonal anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase)
Mouse monoclonal anti-alpha-tubulin

dependent variables

MURC/cavin-4 expression
Cav-3 expression
Myogenin expression
MHC expression
Total and phosphorylated ERK1/2 expression

control variables

GAPDH
Alpha-tubulin

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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