Protocol detail

Multicolor Flow Cytometry for Tumor Cell Isolation

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Connor A.A., Denroche R.E., Jang G.H., Lemire M., Zhang A., Chan-Seng-Yue M., Wilson G., Grant R.C., Merico D., Lungu I., Bartlett J.M., Chadwick D., Liang S.B., Eagles J., Mbabaali F., Miller J.K., Krzyzanowski P., Armstrong H., Luo X., Jorgensen L.G., Romero J.M., Bavi P., Fischer S.E., Serra S., Bakhtiari S.H., Caglar D., Roehrl M.H., Cleary S., Hollingsworth M.A., Petersen G.M., Thayer S., Law C.H., Nanji S., Golan T., Smith A.L., Borgida A., Dodd A., Hedley D., Wouters B.G., O’Kane G.M., Wilson J.M., Zogopoulos G., Notta F., Knox J.J, & Gallinger S. (2019). Integration of Genomic and Transcriptional Features in Pancreatic Cancer Reveals Increased Cell Cycle Progression in Metastases. Cancer cell, 35(2), 267-282.e7.

Publication 2019

BD FACSAria III CD140b PE CD45 PC5

Antibodies Antibody Cd90 Cells Clone Collagenase Cryopreservation Dmso Epcam Fitc Flow cytometry Frozen Nylon Stemcell Tumor

Corresponding Organization : Mount Sinai Hospital

Other organizations : Princess Margaret Cancer Centre, Hospital for Sick Children, SickKids Foundation, Ontario Genomics, McGill University Health Centre, Memorial Sloan Kettering Cancer Center, Mayo Clinic in Arizona, University of Nebraska Medical Center, Mayo Clinic, Massachusetts General Hospital, Sunnybrook Health Science Centre, Health Sciences Centre, Kingston General Hospital, Sheba Medical Center, Tel Aviv University, Occupational Cancer Research Centre

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